All of these options are on the Toolbar tab of Settings.

Toolbar tab of Settings showing Show the floating toolbar, Position, Colour mode, Orientation, Size and a list of keyboard shortcuts.
Settings β†’ Toolbar, with the default settings.
Orientation
Horizontal (the default) or Vertical. The buttons are the same either way; on a vertical toolbar the title bar shows just R.
Size
Small (the default) or Large, for bigger buttons that are easier to see and click.
Colour mode
Black & white (the default), Blue, or Colours, where each group of buttons has its own soft background colour.
Position
Static (the default) leaves the toolbar where you put it β€” top-right of the screen until you drag it somewhere else. Track window makes the toolbar follow the window you’re working in, so it stays close to whatever you’re reading.
Show the floating toolbar
Untick to hide the toolbar. Readable keeps running β€” see Showing and Hiding the Toolbar.
Vertical Readable toolbar with large, colour-coded buttons.
The toolbar set to Vertical, Large and Colours.

Readable remembers your layout and where you put the toolbar between sessions.

Video help

A short video showing this feature in Readable for Mac v2. The video only contacts Vimeo once you press play.

Choosing the toolbar's colours, size and position